The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition. Tested in polyacrylamide gel electrophoresis and Western Blotting. One prominent feature of this protein ladder, is that it remains in the liquid state at the storage temperature therefore, there is not a freeze-thaw cycle involved, making the ladder very stable.Ġ.1~0.4 mg/ml of each protein in 20 mM Trisphosphate (pH 7.5 at 25☌), 2% SDS, 1 mM reducing reagent, 3.6 M Urea, and stabilizer.įor each application, 5-15µl is needed, depending on the size of the wells. No need to heat, dilute, or add reducing agent before loading. The ladder is supplied in protein gel loading buffer and is ready-to-use. It is designed for monitoring protein separated during polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes and for sizing of proteins. The Bullseye Pre-stained Protein Ladder contains 10 prestained proteins, covering a wide range of molecular weights from 10 to 180kDa.
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